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Metabolomics

Renata
dr Magdalena Pajdowska
Laboratory diagnostician
Metabolomics is a branch of science concerned with the study of metabolic processes in the body through the analysis of metabolites. It involves the determination of specific chemical markers that are formed during reactions occurring in the cell in both physiological and pathological states. It allows the detection of metabolic changes resulting from:
  • diseases (oncological, metabolic, cardiovascular, neurodegenerative)
  • exposure to toxic or pharmacological substances
  • changes in diet
Metabolomics is one of the 'omics' fields of biology, alongside genomics, transcriptomics, and proteomics. The first publications in this field appeared in 2002, and it is currently developing rapidly.
Metabolomics uses various analytical methods such as mass spectrometry, gas, or liquid chromatography, which allow the identification of many metabolites and determine their quantitative changes, thus enabling a better understanding of processes occurring in organisms.
Thanks to the vast knowledge and dedication of the Research and Development Department, numerous proprietary methods have been developed and validated for routine diagnostic use. Some methods, such as the determination of tryptophan or arginine metabolite profiles, remain in the realm of scientific collaboration, as their clinical interpretation is not yet known despite our ability to measure them.

List of metabolomic methods:

Abbreviation Method
VITD_DBS Quantitative determination of vitamin D metabolites in DBS by LC-MS/MS
VITD_SP Quantitative determination of vitamin D metabolites in serum/plasma by LC-MS/MS
AA_DBS Quantitative determination of amino acid profile in DBS by LC-MS/MS technique
AA_SP Quantitative determination of amino acid profile in plasma/serum by LC-MS/MS technique
AC_DBS Quantitative determination of acylcarnitine profile in DBS by the LC-MS/MS technique
HCY_DBS Quantitative determination of homocysteine in DBS by LC-MS/MS technique
HCY_SP Quantitative determination of homocysteine in serum/plasma by LC-MS/MS technique
OA_U Qualitative determination of organic acid profile in urine by GC-MS technique
SAICAR_U Qualitative determination of SAICAR in urine by LC-MS/MS technique
P/P_U Quantitative determination of purine and pyrimidine bases profile in urine by the LC-MS/MS technique
TRP_SP Quantitative determination of tryptophan metabolite profile in plasma/serum by LC-MS/MS technique
ARG_SP Quantitative determination of arginine metabolites profile in plasma/serum by LC-MS/MS technique
GSH_DBS Quantitative determination of glutathione index in DBS by LC-MS/MS technique
GSH_WB Quantitative determination of glutathione index in whole blood (EDTA) by LC-MS/MS technique
3-OMD_DBS Quantitative determination of 3-O-methyldopa in DBS by LC-MS/MS technique