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Test objective: To assess the body's vitamin D supply, including specifically:
- differential diagnosis of the causes of rickets and osteomalacia, low-energy fractures unrelated to high-force trauma, i.e., calcium-phosphate disorders
- monitoring of therapy/supplementation with vitamin D preparations
- diagnosis of hypovitaminosis, hypervitaminosis, and vitamin D poisoning
- indications for the determination of 25(OH)D are also intestinal malabsorption, nephrotic syndrome, liver disease, decreased muscle and bone mass, treatment with corticosteroids, antiepileptic drugs, and immunosuppressants.
- Method of testing: High-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)
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Analysed substances:
- 25(OH)D3 (calcifediol, calcidiol, or 25-hydroxy vitamin D3)
- 25(OH)D2 (25-hydroxy vitamin D2)
- 24,25(OH)2D3 (24,25-dihydroxy vitamin D3)
- Epi-25(OH)D3 an empirical form with reduced biological activity – it is measured, but due to lack of clinical interpretation is not presented on the result
- Type of test material: Dried blood spot, serum, plasma.
- Test category: DBS, Metabolomic
Vitamin D metabolite testing from dried blood spot — patient-centric sampling
Masdiag Laboratory offers vitamin D metabolite analysis from dried blood spot (DBS) using validated LC-MS/MS methodology. DBS is a patient-centric sampling approach — the patient collects a few drops of capillary blood at home with a simple finger prick, dries the sample on filter paper, and ships it to our laboratory via standard post. No clinic visit. No cold chain. No venipuncture.
This approach is increasingly adopted by supplement companies, nutraceutical brands, health tech platforms and clinical research organisations as a scalable, convenient alternative to conventional blood testing.
Masdiag participates in the international DEQAS external quality assessment programme, providing independent verification of our vitamin D testing accuracy.
Test material: Dried blood spot (DBS), serum, plasma | Method: LC-MS/MS | ISO 15189 compliant | Available for: individual patients · supplement brands · health tech platforms · CROs · international partners
