Quantitative determination of vitamin D metabolites in serum/plasma by LC-MS/MS technique
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Test objective: To assess the body's vitamin D supply, including specifically:
- differential diagnosis of the causes of rickets and osteomalacia, low-energy fractures unrelated to high-force trauma, i.e., calcium-phosphate disorders
- monitoring of therapy/supplementation with vitamin D preparations
- diagnosis of hypovitaminosis, hypervitaminosis, and vitamin D poisoning
- indications for the determination of 25(OH)D are also intestinal malabsorption, nephrotic syndrome, liver disease, decreased muscle and bone mass, treatment with corticosteroids, antiepileptic drugs, and immunosuppressants.
- Method of testing: High-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)
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Analysed substances:
- 25(OH)D3 (calcifediol, calcidiol, or 25-hydroxy vitamin D3)
- 25(OH)D2 (25-hydroxy vitamin D2)
- 24,25(OH)2D3 (24,25-dihydroxy vitamin D3)
- Epi-25(OH)D3 an empirical form with reduced biological activity – it is measured, but due to lack of clinical interpretation is not presented on the result
- Type of test material: Dried blood spot, serum, plasma.
- Test category: DBS, Metabolomic